removal of iron from mdedia?

Colin Dolphin colin.dolphin at
Thu Oct 5 08:35:02 EST 2000

I would like to prepare iron-depleted LB media. Does anyone know how to
do this? Initially I was going to simply add the specific iron-chelator
deferoxamine which should remove free iron. However, someone pointed out
that, being based on a bacterial siderophore, the bugs might be able to
take up the chelated iron. Is there any form of column support coupled to
this chelator through which I might be able to pass the medium?

Thanks for any help


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