Inclusion body

[Richard P. Grant]

In article 
<Pine.SGI.3.96.1001005145325.7334090B-100000 at mole.bio.cam.ac.uk>, 
Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:

> Scott, one of my collegues recommended sonication to resuspend.  I thought
> it was too violent for a protein.  What do you think?

I sonicate the bugs to crack them anyway.  Can't see that it's a problem 
with protein.

R

-- 
Richard P. Grant MAD Phil      http://www2.mrc-lmb.cam.ac.uk/personal/rpg/ 

Please reply to rpg 'at' mrc-lmb.cam.ac.uk'