help with homoginisation

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Oct 5 09:18:28 EST 2000


In article <rh-0510001235150001 at a6-curtis.ibls.gla.ac.uk>, the eminent
Robert Hartley from the Centre for Cell Engineering wrote
>In article <wL4SHKAikE35UAzE at genesys.demon.co.uk>, Alisha Billington
><a_billington at genesys.demon.co.uk> wrote:
>
>
>CC'd By Email also.
>> Hi there,
>> 
>>         I was wondering if anybody could help, I am trying to grind up
>> samples such as corn etc. to the consistency of flour. But a pestle and
>> mortar introduces to much contamination and needs to be sterilised after
>> every use. I saw somewhere a homogeniser that uses glass beads in an
>> eppendorf tube and grinds up samples to a fine consistency. If anybody
>> else has any information about this I would be grateful to hear from
>> you. 
>> Thanks, Alisha
>
>Hi Alisha.
>
>You may be interested in the beads that are used by the RiboLyser from
>Hybaid (same machine as the Bio101.). 

Too expensive for the few uses we need it for. I was sure that I had
seen an inexpensive one that you basically put a single eppendorf in
with glass beads and shake vigorously. If not it looks like the home
workshop again this weekend!

>Anyway enough of the cheek :-) I'm assuming you want to extract nucleic
>accids or protein and want a completely disrupted cell wall?

That's right.

>PS Genesys? Maybe Duncan can help.
>

I basically forced our new sandwich student to post here, as I was
stuck, as a means of encouraging her to make use of this superb
resource. 


>Regards
>Bob; Calypso User from Sunny Scotland. Very Sunny :-)

Cloudy and miserable South of England.

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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