genomic DNA PCR

clarosa clarosa at biocomp.unl.edu
Thu Oct 5 13:41:56 EST 2000



Justin Hopkins wrote:

> >
> > If DNA amplicon is >500 bp, scale-up the reactions for 50 or 100 uL total
> > volume.
>

The above protocol was pulled from a protocol where the total yield is
important, specifically to produce enough product for sequencing reactions.
Generally the yield drops a bit with longer products, so the rule of thumb
solution was to increase the total volume.   These measures are simply to
conserve reagents.






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