stable peroxide solution for ECL?

[Wolfgang Schechinger]

Lars, the key is to buffer the solution. Commercial preps normallyx 
are stabilized with phosphate buffer (you may have a look on your 30% 
bottle)

Mix 100ul H2O2 (30%) per liter in 0,1M TrisHCL pH 9,35. This
should be stable for month if kept in a non translucent plastic 
bottle in the fridge (we use for convenience empty Amersham ECL kit 
bottles ;-) and add some 10 ul H2O2 evrey few month to regenerate.

All the best, 

Wolfgang





> From:          ronnstrand at my-deja.com
> Subject:       stable peroxide solution for ECL?
> Date:          Fri, 06 Oct 2000 11:19:01 GMT
> Organization:  Deja.com - Before you buy.
> To:            methods at hgmp.mrc.ac.uk

> I would like to make a reasonably stable peroxide solution to be
> used in enhanced chemiluminescence (ECL) experiments (to be stored
> in the refrigerator for, hopefully, months). As I understand urea
> peroxide should be a rather stable compound, but in what buffer
> should I dissolve it in order to keep it stable (ie, which pH is
> suitable?) Grateful for suggestions and comments. Lars
> 
> 
> Sent via Deja.com http://www.deja.com/
> Before you buy.
> 
> 
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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usual disclaimers apply 
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