stable peroxide solution for ECL?
Wolfgang.Schechinger at med.uni-tuebingen.de
Fri Oct 6 06:50:15 EST 2000
Lars, the key is to buffer the solution. Commercial preps normallyx
are stabilized with phosphate buffer (you may have a look on your 30%
Mix 100ul H2O2 (30%) per liter in 0,1M TrisHCL pH 9,35. This
should be stable for month if kept in a non translucent plastic
bottle in the fridge (we use for convenience empty Amersham ECL kit
bottles ;-) and add some 10 ul H2O2 evrey few month to regenerate.
All the best,
> From: ronnstrand at my-deja.com
> Subject: stable peroxide solution for ECL?
> Date: Fri, 06 Oct 2000 11:19:01 GMT
> Organization: Deja.com - Before you buy.
> To: methods at hgmp.mrc.ac.uk
> I would like to make a reasonably stable peroxide solution to be
> used in enhanced chemiluminescence (ECL) experiments (to be stored
> in the refrigerator for, hopefully, months). As I understand urea
> peroxide should be a rather stable compound, but in what buffer
> should I dissolve it in order to keep it stable (ie, which pH is
> suitable?) Grateful for suggestions and comments. Lars
> Sent via Deja.com http://www.deja.com/
> Before you buy.
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
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