FACS detection of myoblasts and fibroblasts
Wolfgang.Schechinger at med.uni-tuebingen.de
Fri Oct 6 07:22:55 EST 2000
I'd like to characterize by FACS analysis a myblast cell culture
derived from a human skeletal muscle biopsy. There probably grow
some fibroblasts, so I have two main cell populations.
As far as I am aware there is no myoblast specific marker, thus I'd
do the following:
1) stain almost all cells with anti MHC-1
2) stain fibroblasts with anti-Thy.1 (CD-90)
1a) stain myoblasts with anti NCAM, since I assume that fibroblasts
will not express this surace antigen
Would someone (more familiar with the matter than I) like to
contribute his/her thoughts?
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
usual disclaimers apply
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