FACS detection of myoblasts and fibroblasts

[Wolfgang Schechinger]

Hi all, 

I'd like to characterize by FACS analysis a myblast cell culture 
derived from a human skeletal muscle biopsy. There probably grow 
some fibroblasts, so I have two main cell populations.

As far as I am aware there is no myoblast specific marker, thus I'd 
do the following:

1) stain almost all cells with anti MHC-1
2) stain fibroblasts with anti-Thy.1 (CD-90)

1a) stain myoblasts with anti NCAM, since I assume that fibroblasts 
will not express this surace antigen

Would someone (more familiar with the matter than I) like to 
contribute his/her thoughts?

Wolfgang


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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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usual disclaimers apply 
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