Pfu

T. Max M. Soegaard tmms at mbio.aau.dk
Fri Oct 6 10:28:58 EST 2000


Oh sorry. I read it as 80 % GC
My mistake
-Max

"T. Max M. Soegaard" wrote:
> 
> Hi.
> You might also try to include betaine in your mix (final 1 M) to reduce
> the difference in melting temp. between AT and GC bp´s.
> Ref would be :
> Hengen P,
> Trends Biochem Sci, vol 22 (1997), pp. 225-226
> and cited refs therein.
> 
> Works for me on GC rich templates (genomic or cDNA).
> -Max
> 
> Ali El-Salanti wrote:
> >
> > Hello.
> > I am having trouble with my Pfu polymerase. Template is genomic DNA and the
> > size of the product should be 2 and 3 kb. With the Taq polymerase I have no
> > problem amplifying the sequence, also when I try smaller bits (like 500 bp)
> > I do get a product with Pfu. But I can not Pfu amplify the large sequnces.
> > Maybe it should be noted that the template i 80 % AT.






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