Inclusion body

Sergio sergioal at bbm1.ucm.es
Fri Oct 6 12:03:03 EST 2000


I always thought that french-press is even harder than sonication and therefore
better for breaking inclusion bodies...
Sergio

"Richard P. Grant" wrote:

> In article
> <Pine.SGI.3.96.1001005145325.7334090B-100000 at mole.bio.cam.ac.uk>,
> Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
>
> > Scott, one of my collegues recommended sonication to resuspend.  I thought
> > it was too violent for a protein.  What do you think?
>
> I sonicate the bugs to crack them anyway.  Can't see that it's a problem
> with protein.
>
> R
>
> --
> Richard P. Grant MAD Phil      http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
>
> Please reply to rpg 'at' mrc-lmb.cam.ac.uk'






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