Inclusion body

Sergio sergioal at
Fri Oct 6 12:03:03 EST 2000

I always thought that french-press is even harder than sonication and therefore
better for breaking inclusion bodies...

"Richard P. Grant" wrote:

> In article
> <Pine.SGI.3.96.1001005145325.7334090B-100000 at>,
> Michael Witty <mw132 at> wrote:
> > Scott, one of my collegues recommended sonication to resuspend.  I thought
> > it was too violent for a protein.  What do you think?
> I sonicate the bugs to crack them anyway.  Can't see that it's a problem
> with protein.
> R
> --
> Richard P. Grant MAD Phil
> Please reply to rpg 'at''

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