sergioal at bbm1.ucm.es
Fri Oct 6 12:03:03 EST 2000
I always thought that french-press is even harder than sonication and therefore
better for breaking inclusion bodies...
"Richard P. Grant" wrote:
> In article
> <Pine.SGI.3.96.1001005145325.7334090B-100000 at mole.bio.cam.ac.uk>,
> Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> > Scott, one of my collegues recommended sonication to resuspend. I thought
> > it was too violent for a protein. What do you think?
> I sonicate the bugs to crack them anyway. Can't see that it's a problem
> with protein.
> Richard P. Grant MAD Phil http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
> Please reply to rpg 'at' mrc-lmb.cam.ac.uk'
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