methylene blue staining of agarose gels

Arnoud van Vliet avvliet at knoware.nl
Fri Oct 6 17:14:41 EST 2000


Hi Marion,

I don't know about using it in gels, but I use to stain transferred RNA (and
DNA) on nylon membranes. I use 0.3% methylene blue in 0.3 M sodium acetate,
stain for 1 min, and destain with water. The staining solution can be reused
a lot of times.
All in all the Ponceau staining for nucleic acids!

hope this helps
Arnoud

"Marion Elflein" <marion.elflein at gmx.de> wrote in message
news:39DD81C3.69904E4F at gmx.de...
> I've been told that it's possible to stain DNA gels with methylene blue
> instead of Ethidium bromide (especially if you need to recover the DNA).
>
> Can anyone send me a protocol of this method?
>
> Thank you,
>
> Marion
>







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