methylene blue staining of agarose gels

Emir Khatipov ekhatipo at
Fri Oct 6 18:19:16 EST 2000

Check Sigma catalog. They sell a 1-L bottle with dry powder to disolve in
water/acetate before use. I guess the dry powder is symply a methylene blue.
I remember borrowing from someone and trying the Sigma's stuff a couple of
year ago, and it worked.

"Marion Elflein" <marion.elflein at> wrote in message
news:39DD81C3.69904E4F at
> I've been told that it's possible to stain DNA gels with methylene blue
> instead of Ethidium bromide (especially if you need to recover the DNA).
> Can anyone send me a protocol of this method?
> Thank you,
> Marion

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