E. coli sans inclusion bodies
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Fri Oct 6 21:11:41 EST 2000
timothy patrick purk <purk at students.uiuc.edu> wrote:
:Do you know of a lab strain that does not have inclusion bodies? or is
:this an urban myth?
If you mean inclusion bodies formed by overexpression of the protein,
then it certainly is a myth. Inclusion bodies are aggregates of
improperly folded/unfolded proteins. Many proteins need help to fold
properly, and E.coli has neither enough of the chaperones to do the
job under condition of huge overexpression, nor these chaperones are
ideally suited for eukariotic proteins. Thus, inclusion body
formation is a function of the protein (and its concentration), not
E.coli strain. There are certainly some subtle variations between
strains, but that's where it all ends.
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