FACS detection of myoblasts and fibroblasts

Tom thomas at steenstrup.org
Tue Oct 10 04:19:06 EST 2000


I seem to remember that the SF/HGF-Receptor may be a marker for satellite
cells. You may want to search medline for more info on that one.

Tom

""Wolfgang Schechinger"" <Wolfgang.Schechinger at med.uni-tuebingen.de> wrote
in message news:200010061222.OAA04537 at intwww.zit.med.uni-tuebingen.de...
> Hi all,
>
> I'd like to characterize by FACS analysis a myblast cell culture
> derived from a human skeletal muscle biopsy. There probably grow
> some fibroblasts, so I have two main cell populations.
>
> As far as I am aware there is no myoblast specific marker, thus I'd
> do the following:
>
> 1) stain almost all cells with anti MHC-1
> 2) stain fibroblasts with anti-Thy.1 (CD-90)
>
> 1a) stain myoblasts with anti NCAM, since I assume that fibroblasts
> will not express this surace antigen
>
> Would someone (more familiar with the matter than I) like to
> contribute his/her thoughts?
>
> Wolfgang
>
>
> -----
> This message is encrypted. Use your brain to decode it.
> -----
> Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
> University of Tuebingen, Germany
> email: wolfgang.schechinger at med.uni-tuebingen.de
> wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
> -----
> usual disclaimers apply
> -----
>
>
> ---







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