blocking transcription in eukariotic cells : how to ?
crepinau at sb-roscoff.fr
Tue Oct 10 09:21:25 EST 2000
We have this cell which is active during its differenciation probably
through a protein present at its surface. I would like to block
transcription in this cell to determine the exact time at which the
protein is transcribed.
If the transcription is blocked and the cell is still active that
means that the protein was already present. I am conscious that
blocking transcription might stop the differenciation and thus
eventhough the protein might be present and stocked somewhere it won't
be exported to the cell surface.
What chemical would you recomand ?
Thank you for reading my message.
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