Positions open, Pharmacia
Simon.Plyte at eu.pnu.com
Wed Oct 11 07:13:02 EST 2000
Pharmacia Corporation, Milan, Italy is seeking talented people to
join our research groups in a multidisciplinary and highly interactive
environment. Pharmacia Corporation is a global company with global
ambitions. We offer competitive salaries, benefits and global career
opportunities limited only by your skill, creativity and performance.
If you are interested in any of the positions described, please send a
CV to Dr. Antonella Isacchi
Phone 0248383791 Email Antonella.Isacchi at eu.pnu.com or Dr Simon Plyte
simon.plyte at eu.pnu.com
Open positions list - Biology Department
Protein Chemist/Mass Spectrometrist
The candidate will be responsible for a Protein Chemistry Unit of 4-5
scientists focused on the analytical characterization of recombinant
proteins to be used for x-ray crystallographic and NMR studies.
These efforts involve domain mapping by limited proteolysis, N- and C-
terminal sequence analysis, and the mapping of phosphorylation and
other post-translational modifications by LCMS and MALDI-TOF
approaches. The candidate will also be responsible for further
development and implementation of instrumentation (MS\MS), and its
applications in conjunction with 2D gels to the identification of the
in vivo substrates of target proteins.
The ideal candidate will have a PhD and 5+ years of experience in
protein mass spectroscopy and other protein chemistry approaches.
Previous experience in the analysis of in vivo protein-protein
interactions will be highly regarded
Biochemist/Molecular Biologist (2 positions).
The candidates will be responsible for the biochemical
characterization of novel molecular targets in Oncology. The
candidates should have a Ph.D. (or equivalent), a strong background in
Biochemistry and Molecular Biology and have experience in the cloning,
expression, purification and biochemical characterization of protein
kinases and their substrates. Further, the candidates should ideally
have experience in kinase assays and enzymology.
Biochemists/Mol. Biologists (2 positions)
The candidates will work in a group dedicated to production and
characterization of proteins for x-ray crystallographic and NMR
studies. The positions require expertise in domain mapping and design
of constructs suitable for structural studies, cloning and expression
of proteins in bacteria, insect and yeast systems and medium-large
scale fermentation and purification technologies. The successful
candidates will possess a PhD (or equivalent) in
Biochemistry/Molecular Biology with 3-5 years of postdoctoral
experience in purification, characterization and crystallization of
proteins. Experience in the production of selenomethionine/isotope
labelled proteins and biochemical approaches to the analysis of
protein phosphorylation is a plus.
The candidate will be responsible for the application of biophysical
approaches to enzymatic assays and protein-protein interaction
studies. The successful candidate will have a PhD in Biophysics and
3+ years of experience in the Biochemical field. The position
requires a strong theoretical background in fluorescence and previous
experience of its applications such as FRET, fluorescence polarization
and time-resolved fluorescence. Protein chemistry knowledge applied to
the labelling of proteins for fluorescence studies is also desirable.
Knowledge of microcalorimetry applied to protein-protein and
protein-ligand interactions is a plus.
More information about the Methods