Complement fixation test kit?

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Wed Oct 11 13:43:40 EST 2000


one common method would be to use an erythrocyte lysis test.
it might be hard to put together depending upon the antigen in question.
and the fact that you can't really buy cobra venom factor anymore
but there are ways to couple antigens to RBCs these cells would then be your
read-out of complement fixation.
goodluck

Student wrote:

> I would like to show that an engineered IgG I have produced can fix human
> complement and thus have effector functions.
>
> "Mark Haynes" <Mark.Haynes at Mail.TJU.Edu> wrote in message
> news:39E36F6F.38660B72 at Mail.TJU.Edu...
> > what exactly to you want to accomplish?
> > eg. are you wondering if sera XYZ has complement or complement fixing
> > antibodies?
> >
> > Student wrote:
> >
> > > Dear All,
> > >
> > > Is anyone aware of any kit that allows me to perform Complement fixation
> > > tests on human IgGs? I am looking for one that doesn't involve
> radioactive
> > > (Cr) if possible. If a kit isn't available, is there a non-radioactive
> > > protocol to perform the same test? Many thanks;.
> >






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