Lambda library of Bacillus - doubt
jakku at mrna.tn.nic.in
Thu Oct 12 01:33:23 EST 2000
I am trying to make a library of Bacillus thuringiensis library in Lambda
DashII vector. But before that I was screening it for the presence of a
particular gene using the gene specific primers. when I do the PCR template
preparation using the quick boiling method for PCR template preparation, I
get the required amplicon, but not with the total DNA prep. I believe that
the gene may lie on a plasmid.
Can anyone point out the mistake I am making here. Can anyone suggest a
good dependable protocol for getting the TOTAL DNA from bacillus
thuringiensis or any other bacillus. sp.
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