Prot./DNA interaction and SDS-PAGE

[Emir Khatipov]

""Michael L. Sullivan"" <mlsulliv at facstaff.wisc.edu> wrote in message
news:v04011701b60cc6b7b43d@[144.92.64.174]...
...
>
> Another possibility for seeing the two forms after IMAC (assuming they are
> his/not his tagged versions) is that the protein forms heteromultimers.
>
> Mike

I did not get the issue of heteromultimers. You mean binging of His/nonHis
to each other? Then boiling in the loading buffer would destroy these
interactions.