Old question: Tm calculation

Konstantin Levitsky klevitsky at biochem.wisc.edu
Fri Oct 13 11:34:49 EST 2000


www.idtdna.com has a free calculator from the DNAStar program.  It will give Tm,
hairpin free energy and self dimer free energy, as well as G+C content.

Kjeld Olesen wrote:

> Hi:
>
> The DNA analysis freeware software pDRAW32 comes with an helperapplication
> called Tm-Calculator (or Olicalc.exe) which will calculate the Tm of
> oligonucleotides (nearest neigbour, GC as well as 2+4 rule methods) and also
> calculate optimum PCR temperature.
>
> pDRAW32 as well as Tm-Calculator can be downloaded from
>
> http://www.geocities.com/acaclone or
> http://www.crosswinds.net/~acaclone/
>
> Best regards,
> AcaClone
>
> QIU Guo Hua wrote in message <39E68691.14AC6F29 at mailhost.jhs.com.sg>...
> >I have 2 primers nearly 40 bp, and I know the (4+2) rule is not suit for
> >them. How can I estimate the Tm of these primers? Thanks.
> >
> >Guohua
> >
> >
> >---

--
Konstantin Levitsky
Biochemistry Graduate Student
Peter J. Belshaw's Group
University of Wisconsin - Madison
---------------------------------
Knowledge Brings Fear.
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