Imidazole quantitation

Paul Cullen Paul.Cullen at
Sun Oct 15 20:07:49 EST 2000

Emir Khatipov wrote:
> Hi,
> Does anybody know how to determine imidazole in solutions - if not
> quantitatively, then qualitatively. I just need to get rid of imidazol used
> to elute my His6 protein from IMAC resin. Imidazol is bad for subsequent
> assays with the protein.
> Thanks
> Emir

I routinely remove imidazol from protein preparations for vaccination by
dialysis against 5L of x1 PBS overnight with one change. I don't know
how to detect if there is any left in my preparations, but these
prepartions are no longer toxic.
Paul Cullen
Bacterial Pathogenesis Research Group
Monash University

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