Site-directed mutagenesis

Student mucineer at iname.com
Mon Oct 16 07:14:18 EST 2000


I 'd save your bucks and use the overlap extension method. It's fast,
efficient and cheap (in your case, only need 2 mutagenesis primers + your 5'
and 3' primer for the gene, which you should have already).


"Malay" <curiouser at ccmb.ap.nic.in> wrote in message
news:002801c0354d$84476800$7b02a8c0 at nic.in...
> Dear Netters:
>
> I have a cloned gene. I want to mutate a single base-pair. Can anyone
> recommend any good protocol. Which kit do you suggest?
>
> Malay
>
> Malay Kumar Basu
> Centre for Cellular and Molecular Biology
> Hyderabad 500007
> I N D I A
>
> Fax: (00-91)40-7171195
> Phone: (00-91)40-7172241
> -----
> Insert inevitable trivial witticism of your choice.
> -----
> curiouser at ccmb.ap.nic.in
>
>
>
>
> ---







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