Fill-in reaction

[Michael L. Sullivan]

I'm not going to spell it out for you, but I personally like to use T4 DNA
polymerase for fill-in reactions.  Because of it's exonuclease activities,
it can blunt either type of overhang you might get with restriction
enzymes.  For detailed protocols to do this, see Molecular Cloning by
Sambrook et al or a similar reference.  If you don't have access to a
general reference like that, you ought to get one ASAP.

Mike


>I would like to know how to do a fill-in reaction.  I am a little "SLOW"
>so I am going to have to have it spelled out to me.
>
>
>have a great day!!!!!!!!
>
>--
>Rodney Earl Pettway.
>University of Florida
>Institute for Food and Agricultural Sciences
>Department of Entomolgy and Nematology
>BLDG 970
>Surge Area Drive
>Gainesville, Florida 32611
>
>
>
>
>---


Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264)-5147 Fax


---