2D electrophoresis forum ???

Warren Gallin wgallin at gpu.srv.ualberta.ca
Mon Oct 16 14:19:20 EST 2000


 Actually, 2D electrophoresis is the most commonly used approach for
doing high throughput proteomics on different tissues/cell cultures. 
There are standardized techniques for doing it and extensive databases
for tentatively identifying proteins based on their position in the gel
and for identifying them bases on mass spec of the spots excised from
the gel.

A good starting point would be to look at the ABRF web site at:

www.abrf.org/

They have an ongoing electronic discussion group and pointers to lots of
handy resources.

Warren Gallin

clarosa wrote:
> 
> 2 d is just a technique that has fallen out of fashion... It is too
> instrument and user specific to be useful to the general community , in my
> opinion, due to the impossibility in reproducing the same results, lab to
> lab.   The 2D community needs a standard way to do things,, otherwise , is
> doomed to obscurity.   Too hard to master,, and once you master the
> technique , everyone moves to a different format!
> 
> "Krzysztof B. Wicher" wrote:
> 
> > Hi Everyone!
> >
> > Do You know if there is a separate forum concerning 2D electrophoresis?
> >
> > If not what do You think about "funding"?
> >
> > Thanks in advance
> >
> > Krzysztof
> >
> > Sent via Deja.com http://www.deja.com/
> > Before you buy.






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