primer quality for sequencing

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Tue Oct 17 03:07:48 EST 2000



Alexander Dobrovic wrote:

> Is it true that HPLC purified primers give better results when used for
> sequencing?

depends on which method you use, or acually may be irrelevant. I'v used
desalted primers for both capillary seqs and traditional radioactive ones.
that the radioactive ones sucked was my own fault. for Abi310 they work
fine.

yours

Susanne






More information about the Methods mailing list