DEPC Vs DMPC

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Tue Oct 17 05:42:51 EST 2000



"Dr. Duncan Clark" wrote:

> In article <39EBED1E.97E783C3 at med.monash.edu.au>, the eminent Paul
> Cullen at Monash University wrote
> >Having never
> >used DEPC I would like to know how to make up DEPC solutions (i.e. do
> >you need to dissolve in an ethanolic solution)
>
> No just stick it straight into the water, leave from 2 hours to O/N then
> autoclave. We autoclave twice just to be safe.

You can do that with DMPC also (1ml per l H2O)

Susanne






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