Ponceau S stain??

[Dr. Hiranya S. Roychowdhury]

At 11:40 PM 10/17/00 -0600, Neal Robert Melvin wrote:
>Does anyone have a protocol for staining nitrocellulose membranes?

1% in 0.1% acetic acid
Soak the NC follwing transfer for 1 min.
Pour off the stain for re-use (can be reused several times).
Rinse off the excess stain with DI water and you'll see the bands clearly.
Red bands against white (Of course!).

>What is the approximate sensitivity threshold for Ponceau?

Not sure; but works fine for most runs. It is slightly higher than CBB-R.

>Can a western always be done after staining with Ponceau?

Yes. Destaining, IMO, is optional, since it occurs during blocking anyway.
The blocking solution (red, afterwards) can be re-used w/o problem.

Photography is also possible! Use incident light. 

BTW, I'm an alumnus of UofC. :)

>Please email me directly as I rarely have newsgroup access...
>
>nrmelvin at ucalgary.ca
>
>"I do not feel obliged to believe that the same God who has endowed us
>with sense, reason, and intellect has intended us to forgo their use. " 
>
>- Galileo Galilei 
>
>Neal Melvin
>University of Calgary - Faculty of Medicine
>Department of Neuroscience - Neuroscience Research Group (NRG)
>Health Sciences Centre
>3330 Hospital Drive, NW
>Calgary, Alberta, Canada
>T2N 4N1
>
>e-mail: nrmelvin at ucalgary.ca
>Phone: (403) 220-4490 (lab)
>       (403) 220-7035 (office)
>       (403) 283-8731 (fax)
>	
>
>
>
>
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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