Best machine for in situ PCR

Rick A. Bright rbright at emory.edu
Wed Oct 18 23:45:39 EST 2000


The licensing status of the thermocycler has no relevance if you use
licensed reagents (which is almost impossible to avoid).


"Joyce Faler" <joycef at uidaho.edu> wrote in message
news:39EE1336.E07AD0BA at uidaho.edu...
> PE is licensed for use with PCR.  Hybaid is not licensed for PCR, and is
> therefore cheaper.  You can purchase the license separately, which will
put
> you back in the same price range as PE.  I'm not familiar with Eppendorf's
> licensing status.
> -Joyce Faler
>
> Neil Taylor wrote:
>
> > Hi, We're in a position to buy a machine for in situ PCR. To start with
I
> > was going to pick the Perkin ElmerGene Amp InSitu machine, but then I
got
> > quotes from Hybaid and Eppendorf (for the Mastercycler gradient + in
situ
> > adaptor) which are both about 30% less expensive.
> >
> > So: Is PE charging extra just for the name?
> > Is it more likely to work using a dedicated in situ machine than a
normal
> > PCR machine with an adaptor stuck on top?
> >
> > I would be grateful for any comments from people with experience of any
of
> > these machines (or any others).
> >
> > Thanks,
> >             Neil
>
>
>
>







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