Best machine for in situ PCR

Rick A. Bright rbright at
Wed Oct 18 23:45:39 EST 2000

The licensing status of the thermocycler has no relevance if you use
licensed reagents (which is almost impossible to avoid).

"Joyce Faler" <joycef at> wrote in message
news:39EE1336.E07AD0BA at
> PE is licensed for use with PCR.  Hybaid is not licensed for PCR, and is
> therefore cheaper.  You can purchase the license separately, which will
> you back in the same price range as PE.  I'm not familiar with Eppendorf's
> licensing status.
> -Joyce Faler
> Neil Taylor wrote:
> > Hi, We're in a position to buy a machine for in situ PCR. To start with
> > was going to pick the Perkin ElmerGene Amp InSitu machine, but then I
> > quotes from Hybaid and Eppendorf (for the Mastercycler gradient + in
> > adaptor) which are both about 30% less expensive.
> >
> > So: Is PE charging extra just for the name?
> > Is it more likely to work using a dedicated in situ machine than a
> > PCR machine with an adaptor stuck on top?
> >
> > I would be grateful for any comments from people with experience of any
> > these machines (or any others).
> >
> > Thanks,
> >             Neil

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