Light Cycler

Paul Cullen Paul.Cullen at
Thu Oct 19 04:05:45 EST 2000

Why does glass in the capillaries stuff up PCR using standard buffers?

We had a light cycler on loan and I found that I could not even
reproduce many reactions that I carry out routinely?

Another interesting question is why does it take more cycles?

Just curious! 

Paul Cullen
Bacterial Pathogenesis Research Group
Monash University

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