jakku at mrna.tn.nic.in
Thu Oct 19 07:56:40 EST 2000
hi.. I have a doubt. Doesn't DNA adhere to glass or do they use siliconised
glass capillaries for the work.
1. Why do they use glass capillaries in the first place when it is so
difficult to handle? Can't they make the capillaries out of some other
tougher materials like teflon or something :-)) (considering the exhorbitant
cost of the coffee machine as sussane nicely puts it).
2. Why is the volume limited to 20 ul. why can't they use larger size
3. How is this process easy, when we have to buy all the buffers and
other paraphernalia only from some particular companies.
----- Original Message -----
From: Paul Cullen <Paul.Cullen at med.monash.edu.au>
To: <methods at hgmp.mrc.ac.uk>
Sent: Thursday, October 19, 2000 2:35 PM
Subject: Light Cycler
> Why does glass in the capillaries stuff up PCR using standard buffers?
> We had a light cycler on loan and I found that I could not even
> reproduce many reactions that I carry out routinely?
> Another interesting question is why does it take more cycles?
> Just curious!
> Paul Cullen
> Bacterial Pathogenesis Research Group
> Monash University
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