Quantifying differential expression

Robyn Roth RRoth at csir.co.za
Thu Oct 19 08:30:42 EST 2000

I have a mutated gene on a plasmid, with the wild-type unmutated gene still active on the chromosome.  Does anyone know of any methods where I can determine the levels of expression of the 2 different genes?  The mutated gene contains a single amino acid change, with a unique restriction enzyme site engineered into it.

Please reply directly to me via e-mail.

Thanks in anticipation,
Robyn Roth
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