Ponceau S stain??
thegrouchybeast at hotmail.com
Thu Oct 19 11:52:08 EST 2000
"Dr. Hiranya S. Roychowdhury" wrote:
> At 11:40 PM 10/17/00 -0600, Neal Robert Melvin wrote:
> >Can a western always be done after staining with Ponceau?
> Yes. Destaining, IMO, is optional, since it occurs during blocking anyway.
> The blocking solution (red, afterwards) can be re-used w/o problem.
> Photography is also possible! Use incident light.
Photocopying also works quite well for Ponceaued blots. Just wrap the
blot in cling-film and photocopy - much easier than taking a photo if
there is a photocopier nearby.
Stained blots can also often be dried out and stored between sheets of
Whatman paper in a box with dessicating crystals, for later Western
processing. This is best done before blocking, because dried blocked
blots are more prone to mold. To continue the Western, simply soak the
blot in PBS-tween for a minute or so to rehydrate then carry on. (NB/
Use this tip at your own peril. Not all antigens may be detectable
after the blot has been dried out, although I've never found it to be a
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