Ponceau S stain??
ekhatipo at NOSPAMmidway.uchicago.edu
Thu Oct 19 14:52:29 EST 2000
I've also been using Amido black die to stain the membranes after blotting.
Same procedure as for Ponceau (0.1% dye in 1% acetic acid). Amido black is
almost the same sensitive as Coomassie, whereas Ponceau is much less
sensitive (moreover, blue stains are better viewable and easier to make a
good photocopy than red).
"Neal Robert Melvin" <> wrote in message
news:Pine.A188.8.131.5210172338580.40866-100000 at acs4.acs.ucalgary.ca...
> Does anyone have a protocol for staining nitrocellulose membranes?
> What is the approximate sensitivity threshold for Ponceau?
> Can a western always be done after staining with Ponceau?
> Please email me directly as I rarely have newsgroup access...
> nrmelvin at ucalgary.ca
> "I do not feel obliged to believe that the same God who has endowed us
> with sense, reason, and intellect has intended us to forgo their use. "
> - Galileo Galilei
> Neal Melvin
> University of Calgary - Faculty of Medicine
> Department of Neuroscience - Neuroscience Research Group (NRG)
> Health Sciences Centre
> 3330 Hospital Drive, NW
> Calgary, Alberta, Canada
> T2N 4N1
> e-mail: nrmelvin at ucalgary.ca
> Phone: (403) 220-4490 (lab)
> (403) 220-7035 (office)
> (403) 283-8731 (fax)
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