lysis E. coli in 96-well plate

Don Walthers donwalthers at hotmail.com
Fri Oct 20 15:33:14 EST 2000


Fisher sells CHCl3-resistant microtiter plates. You can do your lysis in
these plates then transfer to standard plates for subsequent use. Because
they are CHCl3-resistant, they also stand up well to washing and autoclaving
so they can be reused. So I guess your lysis method depends on what you're
trying to get out of the cell. I've used this strategy for b-gal assays.

--
Don Walthers  donwalthers at hotmail.com
BS Microbiology U of Illinois at UC, 1996
PhD Microbiology U of Idaho, soon
Our gene pool needs a little chlorine.







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