Ponceau S stain??
stayve-and-irayne at worldnet.att.net
Sat Oct 21 15:53:47 EST 2000
The Grouchybeast <thegrouchybeast at hotmail.com> wrote in message
news:39EF26B8.75C68689 at hotmail.com...
> Stained blots can also often be dried out and stored between sheets of
> Whatman paper in a box with dessicating crystals, for later Western
> processing. This is best done before blocking, because dried blocked
> blots are more prone to mold. To continue the Western, simply soak the
> blot in PBS-tween for a minute or so to rehydrate then carry on. (NB/
> Use this tip at your own peril. Not all antigens may be detectable
> after the blot has been dried out, although I've never found it to be a
There was a paper in Biotechniques or Analytical Biochemistry a year or so
ago that suggested drying a blot prior to blocking resulted in an increased
signal. Apparently it reduces the loss of antigen from the nitrocellulose
during processing, especially if one blocks in milk which, according to
some, tends to "scrub" antigen off the membrane.
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