How to count labeled protein in SDS-PAGE gel

lxh lxh at dartmouth.edu
Sat Oct 21 17:16:01 EST 2000


Does any know a way to count the radioactivity of proteins from a SDS Gel
stripe? What is the best way to extract the protein or dissolve the gel? I
assume it is not a good idea to count it directly in a scintilation counter.
But the extraction protocol I 've seen takes too many steps, and I am afraid
I will lost some proteins since I only have several microgram protein per
band. Thanks.

lxh








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