rRNA visualization in formaldehyde gels

jph at ibt.dtu.dk jph at ibt.dtu.dk
Mon Oct 23 06:52:10 EST 2000


Hi.

I can not see any rRNA on the formaldehyde gel.

The protocol is the one in Current Protocols : agarose gel, formaldehyde and 
MOPS. The loading buffer is sterile filtrated and then DEPC treated and 
autoclaved. 

Is the detection level in the range 500 ng or can I see like 20 ng?

Must I wash the gel with ammonium?

Any links to RNA gels?

Best regards

Jacob Hofman-Bang, Ph.D
IBT, DTU, Denmark 






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