rRNA visualization in formaldehyde gels

[jph at ibt.dtu.dk]

Hi.

I can not see any rRNA on the formaldehyde gel.

The protocol is the one in Current Protocols : agarose gel, formaldehyde and 
MOPS. The loading buffer is sterile filtrated and then DEPC treated and 
autoclaved. 

Is the detection level in the range 500 ng or can I see like 20 ng?

Must I wash the gel with ammonium?

Any links to RNA gels?

Best regards

Jacob Hofman-Bang, Ph.D
IBT, DTU, Denmark