How to count labeled protein in SDS-PAGE gel

Paul S. Brookes. brookes at uab.edu
Mon Oct 23 08:47:38 EST 2000


Cut the band out using a scalpel, put it in an eppi-tube, and homogenise 
with fresh sample loading buffer containing 5x the normal SDS 
concentration, using one of those disposable pestles.  Then keep at room 
temp' for about an hour, spin down the gel matrix, and load the s/n into 
your scintilation buffer.

Regards
PSB

_________________________________________
Dr. Paul S. Brookes.            (brookes at uab.edu)
UAB Department of Pathology,   G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915     Fax (001) 205 934 1775
http://peir.path.uab.edu/brookes

The quality of e-mails can go down as well as up
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