rRNA visualization in formaldehyde gels
"srohrer(removethis)" at immv.unizh.ch
Mon Oct 23 08:50:54 EST 2000
jph at ibt.dtu.dk wrote:
> The protocol is the one in Current Protocols : agarose gel, formaldehyde and
> MOPS. The loading buffer is sterile filtrated and then DEPC treated and
I hope you do put EtBr into your loading buffer.
> Is the detection level in the range 500 ng or can I see like 20 ng?
more like 500ng-1ug total RNA of which about 70% is rRNA
> Must I wash the gel with ammonium?
not to my knowledge...
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