rRNA visualization in formaldehyde gels

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Mon Oct 23 08:50:54 EST 2000



jph at ibt.dtu.dk wrote:

> The protocol is the one in Current Protocols : agarose gel, formaldehyde and
> MOPS. The loading buffer is sterile filtrated and then DEPC treated and
> autoclaved.

I hope you do put EtBr into your loading buffer.

> Is the detection level in the range 500 ng or can I see like 20 ng?

more like 500ng-1ug total RNA of which about 70% is rRNA

> Must I wash the gel with ammonium?

not to my knowledge...

yours

Susanne






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