Heavy/light chains on Coomassie gel???

Neal Robert Melvin nrmelvin at ucalgary.ca
Tue Oct 24 03:06:25 EST 2000


Hello,

I've been using Coomassie Blue staining to get a rough idea of the
concentration of normal rabbit serum. When I do this, I notice that the
bands corresponding to the heavy chains stain significantly more intensely
than the light chain bands... should these be the rough the same intesity
since one IgG contains 2 heavy chains and two light chains??

I'm trying to match the concentration of this normal rabbit serum to a
known amount of immune rabbit serum so that I can perform an
immunoprecipitation with equivalent amounts of IgG's... which bands should
I try to match in intensity (between my immune serum and my non-immune
serum), the heavy chains of each, or the light chains??

Neal

"If there are many universes, there will be one where there is a set of
numbers suitable to life... we are in that one"

Martin Rees, Astronomer Royal 

Neal Melvin
University of Calgary - Faculty of Medicine
Department of Neuroscience - Neuroscience Research Group (NRG)
Health Sciences Centre
3330 Hospital Drive, NW
Calgary, Alberta, Canada
T2N 4N1

e-mail: nrmelvin at ucalgary.ca
Phone: (403) 220-4490 (lab)
       (403) 220-7035 (office)
       (403) 283-8731 (fax)
	










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