"srohrer(removethis)" at immv.unizh.ch
Tue Oct 24 08:08:52 EST 2000
For the n-th time I have done a Plasmid prep and got a really low yield.
I really hate them. We have tried qiagen, macherey-nagel, both are the
same. What bugs me is how expensive they are. This time I even wasted a
So I've talked to the rep before. First he said to lower the culture
volume (16 instead of 30 ml for a midi), then we find out you're not
supposed to use an overnight culture because the cells are already
starving and chewing up their own plasmids. on the other hand, they tell
you no, you can't use TB.
It just seems to me ages ago they used to work fine. I used to get like
300 ug from a maxi. What (the =*%+) is going on, and what are my
I mean, I get higher yields from a miniprep! Why can't they make their
hydrophobic matrix into maxi columns?!
Susanne in a state of agony...
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