mercapto in transformation?

Gregor Bucher st2153 at
Tue Oct 24 11:38:25 EST 2000

Hi netters,

we compared the stratagene cells to our home made cells and the only
difference came from the "stratagene golden mercaptoethanol" or something
like that. It is a solution you add to the cells before adding DNA. It
also boosts the efficiancy of our home made cells so we would like to make
the solution for ourselves.

Does anybody know what this solution contains? What concentration of
mercapto, what else, pH....


Gregor Bucher, Zoologisches Institut der LMU München
st2153 at

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