Maxiprep woes

Alan Smith alansmith at students.wisc.edu
Tue Oct 24 15:11:58 EST 2000


3) Don't wash with 70% ethanol.

How can not washing with 70% ethanol increase the yield of a DNA prep?  I
was under the impression that DNA was not soluble in 70%.  Sometimes I wash
samples twice with 70% ethanol and have never noticed a loss in expected
yield. I have done about 25 qiagen midipreps and only had a problem when I
overloaded the with DNA, judged by looking at the supernatant from different
steps after the failure.  Maxipreps might however behave differently.

Alan Smith



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