Maxiprep woes

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Oct 25 03:19:40 EST 2000


"Alan Smith" <alansmith at students.wisc.edu> wrote:
> Cornelius Krasel wrote: 
>> 3) Don't wash with 70% ethanol.
> 
> How can not washing with 70% ethanol increase the yield of a DNA prep?

I have often observed that 70% ethanol decreased the yield of a DNA prep.
I don't think that it is because of the DNA being soluble in the ethanol,
but because the pelleting of the DNA after the wash is incomplete.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */






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