mercapto in transformation?

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Oct 25 03:59:06 EST 2000


I remember reading, possibly in the Hanahan paper a long time ago, that
mercaptoethanol was "purified" by them with a flow of nitrogen through the
mercaptoethanol bottle.  I have never tried it, but could it be the way of
obtaining golden mercaptoethanol?  Mike.

On Tue, 24 Oct 2000, Gregor Bucher wrote:

> Hi netters,
> 
> we compared the stratagene cells to our home made cells and the only
> difference came from the "stratagene golden mercaptoethanol" or something
> like that. It is a solution you add to the cells before adding DNA. It
> also boosts the efficiancy of our home made cells so we would like to make
> the solution for ourselves.
> 
> Does anybody know what this solution contains? What concentration of
> mercapto, what else, pH....
> 
> THanks!
> 
> Gregor
> -- 
> Gregor Bucher, Zoologisches Institut der LMU München
> 0049/89/5902/444
> st2153 at zi.biologie.uni-muenchen.de
> ----------------------------------
> 
> 






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