mercapto in transformation?

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Thu Oct 26 02:58:55 EST 2000



Emir wrote:

> 1.42M BME, i.e., 1/10 dilution of concentrated BME. You still have to dilute
> the 1/10 soln 10 times more to use for transformation.
> I guess that the difference of you BME and Stratagene's is that their BME
> same expensive as gold 8-). Just use the purest BME and you'll be fine.

Where did you get that from? I think I'll try that next time. This is for heat
shock cells, if I understood the original post correctly?

Susanne






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