light cycler

[Weining, Song]

In addition to what Duncan and others suggested, you also need to modified
the PCR conditions, mainly reducing the time for each steps. Check Roche or
the agent for why and how. They may supply you the optimisation kit for free
(I used one from Idaho).
No affiliation with these two companies.

Song Weining
Leslie Research Centre
13 Holberton Street
PO Box 2282,
Toowoomba, QLD 4350
Australia

Phone: 61-7-46398880
Fax: 61-7-46398800
Email: weinins at dpi.qld.gov.au


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