bacillus subtilis electroporation

R. Jayakumar jakku at mrna.tn.nic.in
Thu Oct 26 09:03:14 EST 2000


hi..
  anyone know of any good bacillus subtilis electroporation protocols.  I am
trying out the matsuno et al. procedure.  But it is not giving me any
satisfactory results.  Can anyone suggest a good procedure and what are the
electroporation conditions for doing it.  I have a genepulser II aparatus
with me with a pulsecontroller II module attached with it.
   thanking everyone in advance
sincerely
jayakumar



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