phosphorylation of 5' recessed ends

Michael Witty mw132 at
Thu Oct 26 09:41:39 EST 2000

Stewart, in Sambrook et al, they recommend 37 degrees for 15min andthen
45 degrees for 45minutes.  I have used this with success.  Mike.

On 26 Oct 2000 Johns053 at wrote:

> Does anyone know of a method to efficiently phosphorylate 5' recessed ends?  Is
> there a thermostable kinase which could be used for this purpose?
> Many thanks for any suggestions,
> Stewart

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