phosphorylation of 5' recessed ends
mw132 at mole.bio.cam.ac.uk
Thu Oct 26 09:41:39 EST 2000
Stewart, in Sambrook et al, they recommend 37 degrees for 15min andthen
45 degrees for 45minutes. I have used this with success. Mike.
On 26 Oct 2000 Johns053 at mc.duke.edu wrote:
> Does anyone know of a method to efficiently phosphorylate 5' recessed ends? Is
> there a thermostable kinase which could be used for this purpose?
> Many thanks for any suggestions,
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