detergents and gel filtration

Mark Bowen mbo at slac.stanford.edu
Thu Oct 26 08:32:00 EST 2000


Can spomeone point me to literature regarding the behavior of detergent
solutions on gel filration?   Specifically I am interested in
understanding what happens to micelles when the column is run entirely
in solutions above CMC?  Micelles should be separated by size, but above
the CMC they are continuously being added to the column.  Is it best to
run at or close to CMC and assume that protein associated micelles will
be maintatined?

Thanks for any help,

--
Mark Bowen








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