rhubner at gins.uia.ac.be
Fri Oct 27 04:30:57 EST 2000
> T4 ligase protocol has the following modifications
not much difference here IMHO
> > 5ng vector (dephosphorylated)
> > 5ng insert
> 3-10 x more insert than vector
insert is often 3-10 x shorter...
> > 2uL of the 5x ligation buffer
> > 1 U ligase
stock concentrations? unit definitions?
> > total vol. to 10uL
> add 1/10 vol 50% PEG4000 (endconc. 5%)
PEG is included in the buffer described in Focus...
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