native HisTag elution alternative
R. John Lye
rjl6n at virginia.edu
Fri Oct 27 07:04:25 EST 2000
soenke behrends wrote:
> Dear netters,
> we purify a protein containing a HisTAG with
> a Ni-NTA matrix and elute the protein with
> imidazole (250mM). Unfortunately enzyme activity
> of the eluted protein is strongly inhibited by
> 250mM or 150mM imidazole, whereas 50mM imidazole
> lead to 50% of the activity without imidazole.
> We now want to turn to an alternative elution
Is there a reason that you can't dialyze the imidazole away?
More information about the Methods